Hopkins Marine Station Student Paper

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(full paper is archived in the Miller Library)

Title: The blood pigment of the Polychaete annelid Cirriformia spirabancha (Moore, 1904)
Student Author(s): Downs, Isabel
Faculty Advisor(s): Lee, Welton L.
Pages: 17
Location: Final Papers Biology 175H
Date: June 1968
Keywords: Polychaete worm
Cirriformia
Abstract: 1) The blood of Cirriformia spirabrancha showed only one band upon electrophoresis suggesting the presence of only one blood protein. 2) The pigment absorbed at 573 mu, 537 mu, and 400-415 mu in the oxygenated form and at 550 mu and 400-415 mu in the reduced form, the characteristic spectra of oxyhemoglobin and hemoglobin respectively. 3) The molecular weight was estimated at 190,000-200,000. Evidence of fragmentation of the molecule suggest that the weight of 200,000 represents some subunit of a larger molecule. 4) An oxygen-dissociation curve was constructed indicating that the pigment unloads at extremely low tensions of oxygen.