(full paper is archived in the Miller Library)
Title: Inhibition of carbonic anhydrase by DDT and DDE
Student Author(s): Nance, Patrick
Location: Final Papers Biology 175H
Date: June 1969
Abstract: Both DDT and DDE inhibit bovine red cell carbonic anhydrase. Between 3 X 10 ^(-8) and 15 X 10 ^(-8) moles in the assay tube produced 50% inhibition. Homogenated Mytilus mantle appeared to be uninhibited by as high as 3 X 10^(-5) moles of DDT. Mytilus californianus taken from Monterey Bay, California were found to contain 84 ppb of DDT and residues. Sulfanilamide, an additional inhibitor of carbonic anhydrase was capable of inhibiting the mussel enzyme. Only crude homogenates were used in theses studies. Inhibition of mussel carbonic anhydrase should be studied using purified enzyme preparations. DDT (at 3 X 10 ^(-3) M) did not inhibit carbonic anhydrase activity in crude homogenates of the mantle tissue of Mytilus californianus. Sulfanilamide at 3 X 10 ^(-5) M inhibited the Mytilus homogenates. DDT and DDE at 10^(-5) M produce 50% inhibition of bovine red cell carbonic anhydrase.