(full paper is archived in the Miller Library)
Title: A study of the enzymes involved in the excretion of ammonia in the sea anemone Anthopleura elegantissima
Student Author(s): Hetzler, Peter Theodore
Faculty Advisor(s): Phillips, John H.
Location: Final Papers Biology 175H
Date: June 1975
Keywords: sea anemones
Abstract: 1. Fed specimens of the sea anemone, Anthopleura elegantissima, excrete more ammonia than starved specimens, suggesting that A. elegantissima is ammonotelic as expected. 2. Preliminary findings suggest that the endosymbiotic zooxanthellae present in A. elegantissima take up ammonia excreted by the animal. 3. Two steps leading to the excretion of ammonia in A. elegantissima are catalyzed by an L-alanine amidotransferase coupled with a glutamic de-hydrogenase. 4. The level of ammonia released during the course of the L-alanine amidotransferase reaction reaches a maximum at 15 minutes at a rate of 0.13 ug NH3/minute, and thereafter decreases sharply at a rate of 0.04 ug NH3/minute. This decrease cannot be attributed to a glutamine synthetase reaction.