(full paper is archived in the Miller Library)
Title: Freeze-fracture study of the developing morphology of Haliotis rufescens oocytes to nine hours post-fertilization
Student Author(s): Hess, Howard W.
Faculty Advisor(s): Epel, David
Location: Final Papers Biology 175H
Date: June 1988
Keywords: biological studies on gastropods
Abstract: The technique of freeze-fracture in conjunction with scanning electron microscopy (FFSEM) was developed and succesfully used for viewing the developing morphology of the abalone embryo, Haliotis rufescens. The nucleus and intracellular granules/vesicles could be visualized and changes in the distribution of granules followed during embryogenesis. The FFSEM procedure could be further developed as a research tool, and I present some possible improvements in the procedure.