Hopkins Marine Station Student Paper

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(full paper is archived in the Miller Library)

Title: Mitochondrial DNA variation in nearshore rockfishes of Monterey Bay
Student Author(s): Newman, G. Nathan
Faculty Advisor(s): Powers, Dennis
Pages: 34
Location: Final Papers Biology 175H
Date: June 1990
Abstract: Mitochondrial DNA from the livers of adult rockfishes were examined for differences in restriction fragment lengths. After the mitochondrial DNA from kelp rockfish, blue rockfish, black rockfish, and other rockfishes was isolated, it was digested with seven different six-base restriction endonucleases, run on agarose gels, transferred to nylon membranes, probed with radioactive mitochondrial DNA, and the resulting qutoradiographs examined for polymorphisms. We found that all the enzymes showed differences among the three principal species, and that only the kelp rockfish displayed polymorphisms within the species. This method might be used to identify larval rockfish. The results also have interesting implications for the population genetics of the kelp rockfish, S. atrovirens.