(full paper is archived in the Miller Library)
Title: The presence of multi-xenobiotic resistance in marine invertebrates
Student Author(s): Singh, Saween K.
Faculty Advisor(s): Epel, David
Location: Final Papers Biology 175H
Date: June 1991
Abstract: A multi-xenobiotic resistance p-glycoprotein transport activity linked with the multi-drug resistance phenomenon isolated in human tumor cells has been found in various marine invertebrates. Individual cells, assessed by fluorescence microscopy, possess a Rhodamine B efflux activity which can be inhibited by Verapamil, a p-glycoprotein transport inhibitor. In addition, western blot analysis indicates the presence of a protein of a similar molecular weight to the mammalian p-glycoprotein. This activity appears to be a filter feeding lifestyle-linked phenomenon and was found in Urechis caupo eggs, Ascidia ceratodes eggs, and Mytilus californianus gill and mantle tissues. The recognition that marine invertebrates exposed to man-made and natural noxious substances may possess a multi-xenobiotic resistance activity is essential to our understanding of organismal detoxification mechanisms and the biological impact of pollution and could be useful in risk assessment for regulatory policy.