(full paper is archived in the Miller Library)
Title: Immunohistochemical localiztion of serotonin in Melibe leonina
Student Author(s): Timmins, Nathaniel
Faculty Advisor(s): Thompson, Stuart
Location: Final Papers Biology 175H
Date: June 1991
Abstract: Prompted by reports that the application of micromolar concentrations of serotonin to whole animal prepartations of the nudibranch mollusc Melibe leonina increases swimming frequency, an attempt elucidate the specific location and mechanism by which serotonin affects the swemming circuit was undertaken and the first steps have been successfully completed (Morris*, 1990). An immunohistochemical method involving a polyclonal antibody to serotonin and the Avidin-Biotin Complex (ABC) for its visualization has revealed probable endogenous serotonin in numerous locations in the cerebral, tentacular, pedal, and pleural ganglia of Melibe. Preliminary analysis of stained wholemount brains indicates staining on ganglionic and cellular levels, and there is evidence of serotonergic fibers in numerous nerves. Of primary interest is the finding of a cluster three stained cells in the immediate vicinity of a key element of the swimming circuit: Interneuron 1.