Hopkins Marine Station Student Paper

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(full paper is archived in the Miller Library)

Title: Aster formation and cleavage activity in activated nucleate and enucleate sea urchin egg fragments
Student Author(s): Ha, Chung Mea
Faculty Advisor(s): Mazia, Daniel
Pages: 15
Location: Final Papers Biology 175H
Date: June 1991
Abstract: Unfertilized sea urchin eggs, centrifuged into nucleate and enucleate fragments were activated by ammonia and time-lapse videotaped on alight microscope to record movements of the membrane surface. These fragments, unable to divide, exhibit "wiggling" and contractile movement that corresponds in time with the normal first and second mitotic cycles, peaking in intensity with expected cytokinesis. Movement then declines in intensity, corresponding in time with normal interphase. Cytoskeletal isolations performed at times of intense movement show astral organization of microtubules in both nucleate and enucleate fragments.