Hopkins Marine Station Student Paper

Browse Titles | Search Citations & Abstracts

(full paper is archived in the Miller Library)

Title: Developing PCR methodology to assess pollock stocks in the Bering Sea
Student Author(s): Kim, Susan
Faculty Advisor(s): Powers, Dennis
%E Sanders, Sunny
Pages: 24
Location: Final Papers Biology 175H
Date: June 1992
Abstract: Currently, the important pollock fishery in the Bering Sea remains unregulated because of the lack of information regarding its population genetics. PCR amplification and direct sequence analysis provide fisheries biologists with a high resolution method for detecting genetic differences within a species and thus for assessing stocks. The mitochondrial displacement loop (D-loop) gene was targeted for direct sequence analysis due to its elevated rate of mutation. We developed specific PCR methodology for D-loop amplification that involved a superior DNA extraction method using the detergents dodecyltrimethylammonium bromide (DTAB) and cetyltrimethylammonium bromide (CTAB) and a specific pair of primers that amplify the D-loop gene, flanking tRNA genes, and surrounding portions of the 12S rRNA and cytochrome b genes. Overall, we sequenced 200 bases of the surrounding portion of the cytochrome b gene from an Kamchatka Island pollock sample. During an interspecies comparison, 68% of these bases correlated to the same portion of the cytochrome b gene in the shark.