(full paper is archived in the Miller Library)
Title: Expression of GFLN1 sodium channel mRNA at embryonic stages of the squid Loligo opalescens
Student Author(s): Liem, Linda R.
Faculty Advisor(s): Gilly, William F.
Location: Final Papers Biology 175H
Date: June 1993
Abstract: Messenger RNA encording GFLN1, the sodium channel proposed to exist in the giant axon of the squid, was detected in embryos of Loligo opalescens. PCR analysis and RNAse Protection Assay both confirm the initiation of GFLN1 mRNA expression at stage 25 of embryonic development. The level of GFLN1 mRNA expression was found to increase rapidly at stage 27, which immediately precedes giant axon function. Expression levels progressively increased during late development of stages (28-30) which coincide with increased speed of the escape response.