(full paper is archived in the Miller Library)
Title: Dependence of Normal KZ4 Potassium Channel Expression on Microtubule Stability in the Sf9 Cell Line
Student Author(s): Jacobson, Benjamin
Faculty Advisor(s): Gilly, William
Location: Final Papers Biology 175H
Date: June 1995
Abstract: Microtubules are a cytoskeletal, filamentous polymer that have been shown to play a role in fast axonal transport in nerve cells. Further it has been hypothesized that microtubule stability is a factor in determining cell excitability. By culturing Spodoptera frugiperda (Sf9) cells that express the KZ4, shaker potassium channel protein in demecolcine, a microtubule destabilizing drug, it has been shown that there is a link between microtubule stability and the proper expression of the channel through the use of electrophysiology, immuno-flourescence, and immuno-precipitation techniques.