(full paper is archived in the Miller Library)
Title: Short term response of LDH activity to hypoxia in Fundulus heteroclitus
Student Author(s): Morton, John
Faculty Advisor(s): Schulte, Trish
Location: Final Papers Biology 175H
Date: June 1995
Abstract: Previous studies have shown that the activity of lactate dehydrogenase (LDH) in liver of the killifish Fundulus heteroclitus increases approximately two-fold after two weeks of hypoxia. However, nothing is known about the early response of LDH activity to hypoxia. In this study the effects of shorter durations of hypoxia on LDH activities in Fundulus heteroclitus were examined. Hypoxia was induced in aquaria by gassing with an air/nitrogen mixture. After 1, 2, 4, and 7 days of hypoxia fish were killed and livers prepared for LDH assays. LDH activity in liver of Fundulus heteroclitus increased two-fold after two days of hypoxia. This finding demonstrates that the response to hypoxia in Fundulus heteroclitus is much faster than previous research has suggested. These results in F. heteroclitus are consistent with previous findings that mRNA encoding LDH increases after 16 hours of hypoxia in human cell cultures. Possible molecular mechanisms underlying the hypoxia-induced increase in LDH activity are discussed.