Hopkins Marine Station Student Paper

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Title: Nitric oxide promotes while cholinergic agonist and cyclic GMP inhibit neurite outgrowth of N1E-115 mouse neuroblastoma cells
Student Author(s): Shih, Karen
Faculty Advisor(s): Thompson, Stuart
Pages: 29
Location: Final Papers Biology 175H
Date: June 1997
Abstract: The N1E-115 mouse neuroblastoma cell line was used to investigate the role of the cholinergic receptor/nitric oxide/cyclic GMP pathway in regulating neurite outgrowth. Here evidence is presented that both carbachol and 9-Bromo-cGMP inhibit neurite outgrowth. Nitric oxide donors, however, promote various forms of neurite outgrowth. DEANO and Spermine NONOate were shown to promote neurite outgrowth from lamellipodia, but had no effect on neurites that did not possess lamellipodia before administration of the donors. SNAP was shown to allow growth of the main shafts of neurites, but not that of lamellipodia or their extended neurites. This study suggests that nitric oxide promotes growth through a cGMP-independent pathway. During activation of cholinergic receptors, the stimulatory effects of nitric oxide appear to be masked by other pathways in the cholinergic cascade.