Hopkins Marine Station Student Paper

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(full paper is archived in the Miller Library)

Title: Distribution of voltage gated potassium channel beta subunits in the optic lobe of adult, hatchling, and embryonic squid, Lolio opalescens
Student Author(s): Wang, Tammy
Faculty Advisor(s): Gilly, William F.
Pages: 28
Location: Final Papers Biology 175H
Date: June 1997
Abstract: Kvbeta subunits associate with alpha subunits and cause changes in both properties (Kvbeta1) and expression level (Kvbeta2) of Kv potassium channels. Kvbeta1 has been noted to increase channle inactivation rates when co-expressed with Kvalpha. Increases in glycosylation efficiency and surface protein expression of alpha subunits have been observed in the presence of Kvbeta2. A partial Kvbeta cDNA clone (Sqbeta) was isolated form a squid stellate ganglion cDNA library and tentatively identified as a beta2 type. In situ hybridization was used to examine Sqbeta mRNA distribution in aduilt, hatchling, and embryonic squid. In optic lobes, RNA probe specific for Sqbeta preferentially labeled cellular layers containing somata of secondary and tertiary visual neurons, bipolar cells, and large amacrine cells. Based on grain density counts, Sqbeta was present in nearly all medullar cells of post-embryonic squid. In stage 29+ embryos, Sqbeta grain density was noticeable lower than in adults and hatchlins. Kvbeta subunits may be expressed in different cells at various levels during development, adding another level of regulation and possibly playing an important role in increasing Kv channel diversity.