Hopkins Marine Station Student Paper

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Title: The utility of AFLP DNA fingerprinting for the determination of population structure of the market squid Loligo opalescens
Student Author(s): Hayden, Maya
Faculty Advisor(s): Powers, Dennis
Pages: 28
Location: Final Papers Biology 175H
Date: June 1997
Abstract: The market squid Loligo opalescens is distributed along the West coast of North America from Southern Alaska to Baja California. Management of this growing fishery has been difficult due to the lack of knowledge of the population structure of this species. Amplified Fragment Length Polymorphism (AFLP) was thus employed in order to detect the existence of distinct populations along the West coast. The technique (developed by Vos et al., 1995) includes three general steps: digestion of genomic DNA with two restriction enzymes and ligation of oligonucleotide adapters, preamplification of a subset of the restriction fragments, and an additional selective amplification using 33P labeled primers in order to visualize the restriction fragments on an autoradiogram. Preliminary results show the consistency and sensitivity of this technique, as well as an indication of genetic variation among L. opalescens from different locations. However, larger sample sizes would need to be analyzed before any conclusive evidence of population structure can be shown.