(full paper is archived in the Miller Library)
Title: The heat shock protein-70 (Hsp 70) response: differences between subtidal and intertidal groups in a population of Mytilus californianus
Student Author(s): Lopuch, Meredith Ann
Faculty Advisor(s): Somero, George
Location: Final Papers Biology 175H
Date: June 1998
Abstract: Heat shock proteins are one type of molecular chaperone. Their primary responsibility is to help refold proteins that are denatured due to high temperatures. These proteins are essential for organisms living at the edges of their thermal ranges, for example, many intertidal organisms which may be exposed to potentially lethal temperatures during low tide periods. An example of such an intertidal organism is the mussel Mytilus californianus, which is found in both subtidal and intertidal locations, and can often experience temperatures of 30 degrees C and higher. Such temperatures were seen in the two artificial tide pools, one subtidal and one intertidal, which were created for this study. Equal numbers of M. californianus ranging in size were placed in the two pools and samples were collected over five weeks.
Subsequent analysis showed that heat shock protein-70 (Hsp 70) is expressed in response to extreme temperatures. The expressed Hsp 70 was visualized on the gels as two isoform bands - band 1 and band 2. Comparing the mean band 1 and band 2 intensities of Hsp 70 for both subtidal and intertidal groups of M. californianus over five weeks has shown that the intertidal group had elevated levels of Hsp 70 in comparison to the subtidal group for all weeks compared.