Hopkins Marine Station Student Paper

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Title: Intracellular signals trigger metamorphosis in the tunicate Ascidia ceratodes
Student Author(s): Gignoux, Christopher
Faculty Advisor(s): Epel, David
Pages: 22
Location: Final Papers Biology 175H
Date: June 2000
Abstract: The purpose of this experiment was to determine if Ca 2+ and nitric oxide (NO), two common intracellular signaling molecules, affect metamorphosis of the tunicate Ascidia ceratodes. The larvae were assessed for competence to metamorphosis using Nile Blue sulfate, which even at 10-7 M significantly increased settlement. Calcium's affect on settlement was tested using Ionomycin, which induced metamorphosis suggesting calcium's involvement in settlement. Nitroprusside, an NO donor, also induced settlement, suggesting NO's involvement in settlement as well.