(full paper is archived in the Miller Library)
Title: In Vitro growth of zebrafish neural precursors
Student Author(s): Tulloch, Nathaniel
Faculty Advisor(s): Thompson, Stuart
Location: Final Papers Biology 176H
Date: June 2002
Abstract: Because zebrafish continue to grow throughout their adult life, they are constantly generating and differentiating new neural tissue. The periventricular zone (PVZ) of the optic tectum, given its generalized, undifferentiated cell morphology, is a possible source of neural precursor cells whose migration into the tectum and differentiation into several different neural fates may be the source of tectal growth. GFP lipofection of brain cross-slices indicated that at least some of these cells are dividing much of the time. We developed a novel procedure for the isolation of PVC cells and demonstrated growth of neurospheres in culture from these progenitors when driven under various growth factors (Insulin, IGF, Retinoic acid, bFGF). Additionally, preliminary antibody staining suggests that at least a subset of these PVC cells express the Notch 1 receptor in vivo, suggesting the possibility of using this receptor as a specific marker for neural progenitor cells.